2 English reference indigo pulverata levis[ Landau Chinese-English Dictionary]
Indigo Naturalis Powder [Xiangya Medical Dictionary]
Indigo Naturalis [Chinese Medicine Terminology Committee]. Terminology of Traditional Chinese Medicine (2004)]
Indigo Naturalis [Chinese Medicine Terminology Committee]. Terminology of Traditional Chinese Medicine (2004)]
3 overview of indigo naturalis
Indigo Naturalis the name of traditional Chinese medicine, and published the theory of medicinal properties. It is a dry powder or lump made from leaves or stems and leaves of Polygonum multiflorum Thunb. Polygonaceae or Isatis indigotica. Cruciferae [1].
Pharmacopoeia of People's Republic of China (PRC) (20 10 edition) records the pharmacopoeia standard of this Chinese medicine.
4 Latin name indigo naturalis (La) (Chinese medicine terminology (2004))
5 English name indigo naturalis (Chinese medicine terminology (2004))
6 Indigo alias Indigo, Indigo flower, Indigo foam, Indigo.
Indigo, orchid, Indigofera [2].
7. Indigo Naturalis a dry powder, block or granule made by processing the leaves or stems and leaves of mouse. Polygonum multiflorum Thunb. Ait。 Polygonaceae or Isatis indigotica. Cruciferae plants.
Indigo Naturalis a powder made by processing the stems and leaves of Isatis indigotica of Acanthaceae. Or Isatis indigotica of Leguminosae, Isatis indigotica of Cruciferae and Polygonum multiflorum Thunb. [2].
Indigo Naturalis powder or lump made from leaves of Acanthaceae. The original plants for processing indigo naturalis also include the leaves of the wild green tree Indigofera. Polygonum cymosum of Leguminosae. Polygonaceae, Isatis indigotica. Cruciferae plants.
Primitive plants form perennial herbs. The stems are usually branched in pairs, and the tender parts and inflorescences are brown. Leaves opposite, apex acuminate, margin coarsely toothed, black when dry. Spikes erect; Bracts opposite; Calyx 5-lobed; Corolla tubular, purple; 4 stamens, strong enemy; Ovary 2-loculed, 2 ovules per locule. Capsule rod-shaped. The seeds are ovoid. Flowers bloom in autumn and bear fruit in winter.
Born in a wet place on the edge of the forest; Well-bred.
9 Origin Indigo Naturalis mainly produced in Fujian, Yunnan, Guangxi, Guangdong, Jiangxi and Zhejiang.
Indigo Naturalis produced in Hebei, Jiangsu, Anhui, Jiangxi, Fujian, Henan, Sichuan and Yunnan [2].
10 harvesting and primary processing of stems and leaves in summer and autumn, putting them into wooden barrels or tanks, soaking them in water for 2 ~ 3 days and nights, removing branches until the branches and leaves fall off, adding appropriate amount of lime, fully stirring until the immersion liquid changes from dark green to deep purple, removing the blue foam generated on the liquid surface, and drying.
1 1 Pharmacognosy is very fine powder, grayish blue or dark blue, easy to fly, stick to paper, float in water, or porous small pieces. There is a special smell of grass, slightly sour.
12 sexual taste enters the meridians. Indigo Naturalis salty and cold, entering the liver, lung and stomach meridians [2].
13 efficacy and indications of indigo naturalis has the efficacy of clearing away heat, cooling blood and relieving convulsion, and can be used for warming toxin, spotting, vomiting due to blood heat, chest pain, hemoptysis, aphtha, parotitis, laryngitis and infantile convulsion.
Indigo Naturalis has the effects of clearing away heat and toxic materials, cooling blood to stop bleeding, clearing liver and purging fire [2];
Indigo Naturalis used to treat fever, such as heat toxin eruption, blood heat, hematemesis, epistaxis, hemoptysis, liver-heat convulsion, liver-fire and lung cough, etc.: oral, 1.5 ~ 6g, mostly powder [2].
Indigo Naturalis used to treat mumps, otitis media, stomatitis, thrush in children, sore throat, erysipelas, pustulosis and eczema: dry compress or adjust the affected area [2].
The chemical constituents of 14 indigo naturalis mainly contain indigo, indigo and indirubin.
Indigo Naturalis contains indigo and indirubin, which is an effective component in the treatment of chronic myeloid leukemia [2].
15 pharmacological effects of indigo Indigo and indirubin are effective components in the treatment of chronic myeloid leukemia [2].
The ethanol extract of Indigo indigo has inhibitory effect on some bacteria in vitro. This product also has anti-tumor effect [2].
16 the pharmacopoeia standard of indigo naturalis 16. 1 is called indigo naturalis.
Qingdai
Qingdai
16.2 source: this product is dry powder, block or granule made from the leaves or stems and leaves of the mouse. Polygonum multiflorum Thunb. Ait。 Polygonaceae or Isatis indigotica. Cruciferae plants.
16.3 features this product is a dark blue powder, which is easy to fly. Or irregular and porous lumps and particles, are twisted into fine powder by hand. It has a slight smell of grass and a light taste.
16.4 Identification (1) Take a small amount of this product and burn it with a small fire to produce purple smoke.
(2) Take a small amount of this product and drop nitric acid to produce bubbles, which are brownish red or yellowish brown.
(3) Take 50mg of this product, add 5ml of chloroform, fully stir and filter, and take the filtrate as the test solution. In addition, chloroform was added to indigo reference substance and indirubin reference substance to prepare solutions containing 65438±0mg and 0.5mg per 65438±0ml, respectively, as reference substance solutions. According to the test of thin-layer chromatography (Appendix ⅵ b), absorb 5μl of each of the above three solutions, spot them on the same silica gel G thin-layer plate, and use toluene-chloroform-acetone (5: 4: 1) as the developing agent, unfold, take them out and dry them. In the chromatogram of the test sample, the same blue and light purple spots appear in the position corresponding to the chromatogram of the control sample.
16.5 Check that the moisture content of 16.5. 1 shall not exceed 7.0% (appendix ⅸ H first method).
16.5.2 Take 0.5g of this product as water-soluble pigment and add 10ml. Shake the water for a while, and the water layer shall not be dark blue.
16.6 content determination 16.6. 1 indigo was determined by high performance liquid chromatography (appendix 6 d).
Chromatographic conditions and system applicability test with octadecylsilane bonded silica gel as filler; Methanol-water (70: 25) was used as the mobile phase; The detection wavelength is 606 nm. The theoretical plate number should be no less than 1800 in terms of indigo peak.
Preparation of reference substance solution Take 2.5mg of indigo reference substance, weigh it accurately, put it in a 250Wml volumetric flask, and add about 220ml of 2% chloral hydrate chloroform solution (take chloral hydrate, put it in a silica gel dryer for 24 hours, weigh 2.0g, add chloroform to 100ml, let it stand, and if it becomes turbid, dehydrate it with anhydrous sodium sulfate and filter to get it). The frequency is 33 kHz) for 1. 5 hours, cooling, adding 2% chloral hydrate chloroform solution to scale, and shaking well to obtain the product (indigo 10μg per 1ml).
Preparation of test solution Take about 50mg of fine powder of this product, weigh it accurately, put it in a 250ml volumetric flask, add about 220ml of 2% chloral hydrate chloroform solution, perform ultrasonic treatment (power 250W, frequency 33kHz) for 30min, let it cool, add 2% chloral hydrate chloroform solution to scale, shake well, filter and take continuous filtrate.
The determination method accurately absorbs 65438±00ul of the control solution and the test solution respectively, injects them into the liquid chromatograph, and determines them.
This product contains indigo (c16h10no2) which is not less than 2.0% calculated as a dry product.
16.6.2 determination of indirubin by HPLC (appendix ⅵ d).
Chromatographic conditions and system applicability test with octadecylsilane bonded silica gel as filler; Methanol-water (70: 30) as mobile phase; The detection wavelength is 292 nm. Calculated by indirect peak, the theoretical plate number should not be less than 3000.
Preparation of reference solution Take 2.5mg of indirubin reference substance, weigh it accurately, put it in a 50ml volumetric flask, add about 45 ml of N, N dimethylformamide, ultrasonically treat it (power 250W, frequency 33kHz) to dissolve it, let it cool, add N, N dimethylformamide to the scale, and shake it evenly; Accurately measure 100ml, put it in a 100ml volumetric flask, add N, N dimethylformamide to the scale, and shake well to get the product (containing 5μg of indirubin per 1ml).
Preparation of test solution Take about 50mg of this fine powder, weigh it accurately, put it in a 25ml volumetric flask, add about 20ml of N, N dimethylformamide, perform ultrasonic treatment (power 250W, frequency 33kHz) for 30min, cool it, add N, N dimethylformamide to the scale, shake it evenly, filter it, and take the filtrate.
The determination method accurately absorbs 65438 00μ l of the control solution and the test solution respectively, and injects them into a liquid chromatograph for determination, thus obtaining the product.
The content of indirubin (c16h10no2) in this product is not less than 0. 13% on a dry basis.
16.7 sexual taste and salty and cold meridian tropism. Liver meridian.
16.8 Functions and indications: clearing away heat and toxic materials, cooling blood and removing spots, purging fire and relieving convulsion. Can be used for treating fever, blood-heat vomiting, chest pain and hemoptysis, aphtha, parotitis, laryngitis, infantile convulsion and epilepsy.
16.9 Usage and dosage: 1 ~ 3g, Yiren Pill is for casual use. Appropriate amount for external use.
16. 10 Store in a dry place.
16. 1 1 People's Republic of China (PRC) Pharmacopoeia (version 20 10)
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