Contents 1 Pinyin 2 English reference 3 Overview 4 Latin name 5 English name 6 Pharmacopoeia standards for the preparation of Sichuan Aconite 6.1 Product name 6.2 Source 6.3 Preparation method 6.4 Properties 6.5 Identification 6.6 Inspection 6.6.1 Moisture 6.6.2 Diesters Type biotin 6.7 Content determination 6.7.1 Chromatographic conditions and system suitability test 6.7.2 Preparation of reference solution 6.7.3 Preparation of test solution 6.7.4 Determination method 6.8 Nature, taste and meridians 6.9 Function and indication 6.10 Usage and dosage 6.11 Note 6.12 Storage 6.13 Source 7 Attached reference materials: 1 Prescriptions using traditional Chinese medicine to prepare Sichuan Aconite 2 Chinese patent medicines using traditional Chinese medicine to prepare Sichuan Aconite 3 Preparation of Sichuan Wu in ancient books 1 Pinyin
zhì chuān wū 2 English reference
preparemonkshd moter root [Xiangya Medical Professional Dictionary] 3 Overview
Preparation and processing of the dried mother root of Aconitum carmichaeli Debx. of the Ranunculaceae plant. Product[1]. 4 Latin name
Radix Aconiti Preparata (La) ("Terms of Traditional Chinese Medicine (2004)") 5 English name
prepared mon monkshood mother root ("Terms of Traditional Chinese Medicine (2004)") )
6 Pharmacopoeia standard 6.1 for the preparation of Aconite root
Zhichuanwu preparation
Zhichuanwu
ACONITI RADIX COCTA 6.2 Source
This product is a processed product of Sichuan Wu. 6.3 Preparation method
Take Sichuan Aconite, separate into large and small pieces, soak in water until there is no dry core inside, take it out, add water and boil for 4 to 6 hours (or steam for 6 to 8 hours) until the large and solid ones are cut into pieces. When there is no white heart inside and there is a slight numbing sensation in the mouth, take it out, dry it until it is 60% thick, slice it and dry it. 6.4 Properties
This product is an irregular or elongated triangular piece. The surface is dark brown or yellowish brown, with gray-brown cambial ring patterns. The body is light, brittle, and the cross section is shiny. The breath is weak and the tongue feels slightly numb. 6.5 Identification
Take 2g of this product powder, add 2ml of ammonia test solution to moisten it, add 20ml of ether, ultrasonic treatment for 30 minutes, filter, recover the solvent at low temperature until dry, add 1ml of methylene chloride to the residue to dissolve, as Test solution. In addition, take an appropriate amount of benzoyl aconitum original reference substance, benzoyl aconitum original reference substance and benzoyl aconitum original reference substance, add isopropyl alcohol chloroform (1:1) to dissolve and prepare A mixed solution containing 1 mg of each in 1 ml was used as the reference solution. According to the thin layer chromatography (Appendix VI B) test, absorb 5 ul of each of the above solutions and spot them on the same silica gel G thin layer plate. Use n-hexane ethyl acetate methanol (6.4:3.6:1) as the developing agent and saturated ammonia vapor. After 20 minutes, unfold, take out, dry, and spray with dilute potassium bismuth iodide test solution. In the chromatogram of the test product, spots of the same color appear at the corresponding positions in the chromatogram of the reference substance. 6.6 Inspection 6.6.1 Moisture content
shall not exceed 11.0% (Appendix IX H First Method). 6.6.2 Diester type biological salts
Test according to the chromatographic conditions and preparation method of the test solution according to [content determination item].
Preparation of reference solution? Take appropriate amounts of Aconitum reference substance, secondary Aconitum reference substance and new Aconitum reference substance, weigh them accurately, and add isopropyl alcohol chloroform (1:1 ) mixed solution was dissolved to prepare a mixed solution containing 50 μg of Aconitum, 0.15 mg of Aconitum and Neoaconitum per ml.
Measurement method? Precisely draw 10 μl each of the reference solution and the test solution under [Content Determination], inject them into the liquid chromatograph, and measure.
This product contains diester-type biological alkaloids based on the total amount of Aconitum (C34H47NO11), Second Aconitum (C33H45NO10) and New Aconitum (C33H45NO11), which shall not exceed 0.040%. 6.7 Content determination
Determine according to high performance liquid chromatography (Appendix VD).
6.7.1 Chromatographic conditions and system suitability test
Use octadecylsilane bonded silica gel as the filler; use acetonitrile tetrahydrofuran (25:15) as the mobile phase A, 0.1mol/L ammonium acetate ( Add 0.5ml of glacial acetic acid per 1000ml) as mobile phase B, and perform gradient elution as specified in the following table; the detection wavelength is 235nm. The number of theoretical plates should not be less than 2,000 based on the new Aconitum peak. Time (minutes) Mobile phase A (%) Mobile phase B (%) 0～48 15→26 85→74 48～49 26→35 74→65 49～58 35 65 58～65 35→15 65→85 6.7. 2 Preparation of reference solution
Take appropriate amounts of benzoyl Aconitum original reference substance, benzoyl Aconitum original reference substance and benzoyl Aconitum original reference substance and weigh them accurately. , add isopropanol and chloroform (1:1) solution to dissolve to prepare a solution containing 50 μg of benzoyl aconitum and benzoyl aconitine 0.3 mg per 1 ml. Mix the solution and get it. 6.7.3 Preparation of test solution
Take about 2g of the powder of this product (passed through No. 3 sieve), weigh it accurately, place it in a stoppered conical flask, add 3ml of ammonia test solution, and accurately add different 50ml of the mixed solution of propanol and ethyl acetate (1:1) was weighed and treated with ultrasonic treatment (power 300W, frequency 40kHz; water temperature below 25°C) for 30 minutes, let cool, weighed again, and treated with isopropyl alcohol and ethyl acetate. Make up the lost weight with a mixed solution of ester (1:1), shake well, and filter. Precisely measure 25 ml of the additional filtrate, recover the solvent under reduced pressure below 40°C until it is dry, and accurately add 3 ml of isopropanol and chloroform (1:1) mixed solution to the residue to dissolve, plug tightly, shake well, filter, and take the additional filtrate, that is have to. 6.7.4 Determination method
Precisely draw 10 μl each of the reference solution and the test solution, inject them into the liquid chromatograph, and measure.
Calculated as dry product, this product contains benzoyl aconitum (C32H45NO10), benzoyl aconitum (C31H43NO9), and benzoyl aconitum (C31H43NO10). The total amount should be 0.07% ~ 0.15%. 6.8 Nature, flavor and meridians
Pungent, bitter, hot; poisonous. Guixin, liver, kidney, spleen meridians. 6.9 Functions and Indications
Expelling wind and removing dampness, warming menstruation and relieving pain. It is used for wind-cold dampness, joint pain, cold pain in the heart and stomach, cold hernia pain and anesthesia to relieve pain. 6.10 Usage and dosage
1.5~3g, fry first and then fry for a long time. 6.11 Note
Pregnant women should use with caution; not suitable for use with pinellia, Trichosanthes trichosanthes, Trichosanthes trichosanthes seeds, Trichosanthes trichosanthes peel, Trichosanthes trichosanthes, Fritillaria chuanensis, Fritillaria fritillaris, Fritillaria flatifolia, Fritillaria japonica, Fritillaria fritillaris Hubei. , Bai Qian, Bai and can be used together. 6.12 Storage
Store in a ventilated and dry place to prevent moth.
6.13 Source