Sterilization is to kill the original bacteria in the culture medium or miscellaneous bacteria attached to the air, so that the target bacteria can grow better (reducing the consumption of nutrients in the culture medium by miscellaneous bacteria, and some miscellaneous bacteria will inhibit the growth of the target bacteria). Generally, after sterilization, the culture medium is cultured at 30-37 degrees for one day to see whether there are long bacteria (whether the liquid becomes turbid or whether the solid grows colonies), that is, whether it is sterile after sterilization.
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Precautions:
The container used for heating, dissolving or high-temperature sterilization of the culture medium should not be a copper pot or an iron pot, so as to avoid a trace of copper or iron mixed into the culture medium and make it difficult for bacteria to grow. Stainless steel pot is best used for heating and melting. Can be put into a beaker or flask and cooked and melted in an autoclave or a flowing steam sterilizer. After being dissolved in the pot, it can be heated with warm water and stirred at any time to prevent coking. If coking is found, the culture medium cannot be used and needs to be reconstituted.
The culture medium should be packed in appropriate containers, such as test tubes and flasks, according to the purpose and requirements of use. The quantity of repackaging shall not exceed 2/3 of the container capacity. The mouth of the container can be plugged with a cotton plug padded with moisture-proof paper, and the outside of the container must be wrapped with waterproof paper (at present, test tubes are generally covered with nuts).
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