The biggest turning point of the three countries

If it is the three kingdoms in a broad sense (the time period in the romance of the three kingdoms), then the biggest turning point is the yellow turban insurrectionary chaos, which leads to the existence of the Eastern Han Dynasty in name only and the disputes among princes.

If it is the Three Kingdoms in a narrow sense (Cao Pi in the Western Jin Dynasty replaced the Han Dynasty to destroy Dongwu), then the biggest turning point is the battle of Yiling, which caused heavy losses to Wu and Shu, and even if the two countries were reunified later, it could not pose a threat to Cao Wei.