1. experimental purpose: through practical operation, master the general operation methods of rats and mice, including grasping and fixing, sex identification, drug administration and blood collection of rats and mice.
2. Experimental animals: 4 Kunming mice (half male and half female), 4 rats (half male and half female), 2 gastric lavage devices, 4 syringes, alcohol, cotton balls, normal saline, 1 mouse fixator, 1 rat fixator.
Third, the experimental steps
1, grab and repair
1. 1 Grab: Grab the tail root of the mouse with your left hand.
1.2 fixation: Hold the tail root of the mouse with the left hand, let the mouse crawl on the rough surface, and then pull the tail heel. The thumb and forefinger of the right hand hold the skin of the neck between the ears of the mouse, and the little finger and ring finger fix the tail on the surface of the palm.
1.3 The rats were caught and fixed in the same way.
2. Gender identification:
2. 1 Grab and fix the mouse
2.2 Observe the distance between anus and genitals and the hair between them. Male: Long distance, dense hair (like other parts); Female: Short distance and sparse hair.
2.3 Repeat the same identification method to identify rats.
take medicine
3. 1 gavage method
3. 1. 1 Grasp and fix the mouse with the left hand in the right way, so that the abdomen is up and the neck is straight.
3. After1.2 is fixed, hold the syringe connected with the intragastric needle in the right hand to suck out the liquid medicine (or suck out the liquid medicine in advance).
Suction), insert the needle into the mouth from the corner of the mouth, then press the head with a gastric lavage needle to make the mouth and esophagus in line, then gently enter the gastric lavage needle along the palatal wall, rotate the needle to stimulate the animal to swallow, and then slowly insert it into the esophagus along the posterior pharyngeal wall.
…………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
Chapter II: Animal Experiment Report
General operating techniques of animal experiments (mice)