2 English reference Zheng Gu mixture [Chinese medicine terminology committee. Terminology of Traditional Chinese Medicine (2004)]
Water [Chinese Medicine Terminology Committee]. Terminology of Traditional Chinese Medicine (2004)]
Overview Bone-setting water is a Chinese patent medicine, and its main components are Jiulongchuan Nature Reserve Nature Reserve, Radix Aucklandiae, Caulis Piperis, Eupolyphaga Seu Steleophaga, Douchi Ginger, Fructus Gleditsiae Abnormalis, Cortex Periplocae, Rhizoma Curcumae, Corn Stalk, Caulis Polygoni Multiflori, Cortex Cinnamomi Japonici, Xu Changqing, Lignum Dalbergiae Odoriferae, Radix Zanthoxyli, Rhizoma Polygoni Cuspidati, Caulis Sinomenii, Radix Flemingiae Japonicae, and Rhizoma Polygoni Cuspidati. Has the effects of promoting blood circulation, removing blood stasis, relaxing muscles and tendons, activating collaterals, and relieving swelling and pain. It can be used to relieve falls and sprains, fractures and dislocations, and fatigue before and after physical exercise.
4. Pharmacopoeia Standard for Bone-setting Water 4. 1 Name of Bone-setting Water
water
4.2 Prescription Jiulongchuan Nature Reserve Nature Reserve, Radix Aucklandiae, Caulis Piperis, Eupolyphaga Seu Steleophaga, Douchi Ginger, Fructus Gleditsiae Abnormalis [2], Cortex Periplocae, Rhizoma Curcumae, Stigma Maydis, Caulis Polygoni Multiflori, Cortex Cinnamomi Japonici, Xu Changqing, Lignum Dalbergiae Odoriferae, Radix Zanthoxyli, Rhizoma Polygoni Cuspidati, Caulis Sargentodoxae, Radix Flemingiae, Ardisiae, and Rhizoma Paridis.
4.3 The preparation of the above-mentioned twenty-six spices, except Xu Changqing, Zanthoxylum nitidum, Dalbergia odorifera, menthol, camphor and some five-flavored vines, put the remaining twenty spices and the remaining five-flavored vines into an extraction tank, add 1000ml of ethanol and appropriate amount of water, seal, heat and reflux for 7 hours, then distill and collect about 1200ml of distillate. Respectively pulverizing Xu Changqing, Zanthoxylum nitidum, Lignum Dalbergiae Odoriferae and other five kinds of rattan into coarse powder, adding into the above distillate, stirring uniformly, and soaking for 48 hours. Take the soaking solution, add menthol and camphor, stir and dissolve, filter, and adjust the total amount to 1000ml.
4.4 Characteristics This product is a brown-red clear liquid; The breath is fragrant.
4.5 Identification (1) Take this product 15ml, put it in a separating funnel, add 25ml of petroleum ether (30 ~ 60℃), shake and extract, separate the lower liquid, evaporate it in water bath, add 4ml of 75% ethanol to the residue to dissolve it, and filter the filtrate as the test solution. Another 65438 0 g of Dalbergia Odorifera was soaked in 65438±00ml of 75% ethanol for 30 minutes, filtered, and the filtrate was concentrated to 2ml as a control medicinal solution. According to the test of thin-layer chromatography (appendix ⅵ b of Pharmacopoeia I, 20 10), absorb 3μl of the above two solutions, respectively spot them on the same silica gel G thin-layer plate, use chloroform-methanol-concentrated ammonia solution (90: 9: 1) as the developing agent, unfold, take them out, dry them in the air, and place them in an ultraviolet lamp (365nm). In the chromatogram of the test sample, the spots with the same color appear at the positions corresponding to the chromatogram of the control medicinal materials.
(2) Take this product as the test solution. In addition, paeonol reference substance was added with 60% ethanol to make a solution containing 0.5mg per 1ml, which was used as a reference substance solution and was tested by thin layer chromatography (Appendix VI B of Pharmacopoeia I, 20 10). Suck 10μl of test solution and 5μl of reference solution, respectively, and spot them on the same silica gel G thin-layer plate, using benzene-ethyl acetate (20: 1) as developing agent, unfold, take them out, dry them in the air, spray them with hydrochloric acid acidic 5% ferric chloride ethanol solution, and heat them until the spots are clear. In the chromatogram of the test sample, spots with the same color appear in the position corresponding to the chromatogram of the control sample.
(3) Take 65438 05 ml of this product, evaporate it to dryness, and add 65438±0ml of ethanol to the residue to make it dissolve, and use it as the test solution. In addition, 65438 0 g of Zanthoxylum nitidum was taken, 65438 05 ml of ethanol was added, ultrasonic treatment was carried out for 30min, filtration was carried out, the filtrate was evaporated to dryness, and the residue was dissolved in 65438±0ml of ethanol as a control medicinal solution. According to the test of thin-layer chromatography (Appendix ⅵ b of Pharmacopoeia I, 20 10), absorb 4μl of the above two solutions, respectively spot them on the same silica gel G thin-layer plate, use toluene-ethyl acetate-methanol (25: 2: 0. 1) as the developing agent, unfold, take them out, dry them in the air, and place them under an ultraviolet lamp (330. In the chromatogram of the test sample, fluorescent spots with the same color appear at the positions corresponding to the chromatogram of the control medicinal materials.
(4) Take 65438 0 ml of this product and add ethanol to 50 ml as the test solution. In addition, camphor reference substance and menthol reference substance were added with ethanol to prepare solutions containing 65,438+0 mg per 65,438+0 ml respectively, which were used as reference substance solutions and determined by gas chromatography (Appendix VI E, Pharmacopoeia I, 2065,438+00). The column length is 3m, and polyethylene glycol 20000(PEG20M) is used as the stationary phase, and the coating concentration is 65,438. Absorb the above three solutions respectively and inject them into the gas chromatograph. The test sample should present the same chromatographic peak as the control sample.
4.6 Check that the ethanol content in 4.6. 1 should be between 56% and 66% (Appendix ⅸ m of Pharmacopoeia 20 10).
4.6.2 Others shall comply with the relevant provisions under tincture (China Pharmacopoeia, 20 10, Appendix I N).
4.7 Content determination 4.7. 1 Accurately measure volatile oil 10ml, put it in a separating funnel, add 100ml saturated sodium chloride solution, shake it evenly for1~ 2min, let it stand for1~ 2hr, separate the upper liquid and transfer it to a round-bottomed flask.
4.7.2 Xu Changqing was determined by high performance liquid chromatography (China Pharmacopoeia, 20 10, Appendix VI D).
4.7.2. 1 chromatographic conditions and system applicability test, using octadecylsilane bonded silica gel as filler; The mobile phase was acetonitrile-water-triethylamine-phosphoric acid (28: 72: 0.1:0.1); The detection wavelength is 274 nm. The theoretical plate number should be not less than 3000 calculated by paeonol peak.
Preparation of 4.7.2.2 Reference Solution Take appropriate amount of paeonol reference substance, weigh it accurately, and add ethanol to make a solution containing 60μg per 1ml.
Preparation of 4.7.2.3 test solution Accurately measure 65438±00ml of this product, put it in a 25ml volumetric flask, add ethanol to dilute to scale, and shake well to get the final product.
4.7.2.4 method accurately absorbs 65438 00μ l of control solution and test solution respectively, and injects them into liquid chromatograph for determination.
The Xu Changqing content of this product is calculated as paeonol (c9h1oo3) per 1ml, and shall not be less than 0. 10mg.
4.8 Functions and indications: promoting blood circulation to remove blood stasis, relaxing muscles and tendons, relieving swelling and pain. It can be used to relieve falls and sprains, fractures and dislocations, and fatigue before and after physical exercise.
4.9 Usage and dosage: Gently rub the affected area with medicated cotton dipped in liquid medicine; In severe cases, apply cotton wool soaked in medicinal liquid to the affected area for 65438 0 hours, 2 ~ 3 times a day.
4. 10 Pay attention to avoid oral administration; Can't rub into the wound; If itching and rash occur during taking the medicine, please stop using it.
4. 1 1 specification per bottle? ( 1) 12ml？ (2) 30ml？ (3)45 ml (4)88 ml
4. 12 stored in a cool place and sealed.
4. 13 edition of China Pharmacopoeia 20 10 edition.
5. Chinese medicine standard for bonesetting water 5. 1 pinyin name water
5.2 standard number WS3B338798
5.3 Prescription: Jiulongchuan Nature Reserve Nature Reserve, Radix Aucklandiae, Caulis Sinomenii, Eupolyphaga Seu Steleophaga, Radix Aucklandiae, Fructus Gleditsiae Abnormalis, Cortex Acanthopanacis, Rhizoma Curcumae, Herba Polygoni Multiflori, Radix Zanthoxyli, Caulis Polygoni Multiflori, Caulis Sinomenii, Radix Flemingiae, Herba Polygoni Multiflori, Radix Stephaniae Tetrandrae and Eagle.
5.4 Characteristics This product is a brown-red clear liquid; The breath is fragrant.
5.5 Identification (1) Take a few drops of this product on the filter paper, and after it evaporates, check it under the ultraviolet lamp (365nm), showing light yellow fluorescence.
(2) Take this product 15ml, put it in a separatory funnel, add 25ml of petroleum ether (30 ~ 60℃), shake it evenly, let it stand, separate the lower solution, dry it in a water bath, add 4ml of 75% ethanol to the residue to dissolve it, and filter it. The filtrate was used as the test solution. In addition, 65438 0 g of Dalbergia Odorifera was taken, and 65438±00ml of 75% ethanol was added, soaked for 30min, filtered, and the filtrate was concentrated to about 2ml in water bath, which was used as the control medicinal material solution. According to the thin-layer chromatography test (Appendix ⅵ b), suck 3μl of the above two solutions, respectively, and spot them on the same silica gel G thin-layer plate with sodium carboxymethyl cellulose as adhesive, and use chloroform-methanol-concentrated ammonia solution (90:9: 1) as developing agent, and spread them with the spreading distance of 10cm, then take them out, dry them and put them in purple. In the chromatogram of the test sample, fluorescent spots with the same color appear at the positions corresponding to the chromatogram of the control medicinal materials.
5.6 Check that the amount of ethanol should be 56 ~ 66% (Appendix ⅸ m).
Accurately absorb 10ml volatile oil, put it in a separatory funnel, add 100ml saturated sodium chloride solution, shake it evenly for1~ 2min, let it stand for1~ 2hr, and transfer the supernatant to a round-bottomed flask. Wash the separating funnel with hot water for several times, combine the washing liquids in a round-bottomed flask, and determine the volatile oil content according to the volatile oil determination method (Appendix X D A), and the volatile oil content shall not be less than 9.5%.
Absorb 25ml of this product from the total solid, put it in an evaporating dish that has been dried to constant weight, evaporate it in a water bath, put it in a silica gel dryer for 24 hours, take it out, accurately weigh it, and the residue shall not be less than 1.0%.
Others shall comply with the relevant provisions under tincture (Appendix I N).
5.7 Functions and indications: relaxing muscles and activating collaterals, removing blood stasis and relieving pain, expelling wind and removing dampness. Used for falls, sprains and various fractures.
5.8 Usage and dosage: Gently dip the cotton wool into the affected area, and apply it to the affected area for 65,438+0 hours, 2 ~ 3 times a day in severe cases.
5.9 Pay attention to avoid oral administration; Don't rub people's wounds; If itching and rash occur during taking the medicine, please stop using it.
5. 10 specifications per bottle
(1)12ml
(2)15ml
(3)30 ml
5. 1 1 Store in a cool place.
Draft by Guangxi Zhuang Autonomous Region Institute for Drug Control
6. Instructions for Jiegu Water 6. 1 drug name Jiegu Water
6.2 tincture dosage forms: each bottle12ml, 30ml, 45ml and 88ml.
6.3 The main components of bonesetting water are Jiulongchuan Nature Reserve Nature Reserve, Eupolyphaga Seu Steleophaga, Rhizoma Curcumae, Polygonum cuspidatum, Xu Changqing, Polygonum Multiflori Radix, Radix Stephaniae Tetrandrae, Camphor, Daphne Daphne, Radix Aconiti Kusnezoffii, Mentholum, etc.
6.4 The function of bonesetting water is to promote blood circulation and remove blood stasis, relax muscles and tendons, and relieve swelling and pain. Can be used for traumatic sprain, various fractures and dislocations, and can be applied before and after exercise to relieve fatigue.
6.5 Precautions 1. Occasionally, skin itching and rashes occur. If itching and rash occur during medication, please stop using it.
2. Avoid oral administration.
The medicine can't rub into the wound.
6.6 Usage and dosage of bonesetting water