2 English reference Lonicera japonica Thunb [Landau Chinese-English Dictionary]
Lonicera japonica Thunb [Xiangya Medical Dictionary]
Gold and silver stems [Xiangya Medical Dictionary]
Lonicera japonica Thunb [Xiangya Medical Dictionary]
Lonicera japonica [Xiangya Medical Dictionary]
Lonicera japonica Thunb. Terminology of Traditional Chinese Medicine (2004)]
Gold and silver flower stems [Chinese medicine terminology Committee] Terminology of Traditional Chinese Medicine (2004)]
3. Overview of honeysuckle. Correcting the name of traditional Chinese medicine and giving a collection of notes on the compendium of materia medica. It is the stem of Lonicera japonica Thunb. [1] of Caprifoliaceae.
Pharmacopoeia of People's Republic of China (PRC) (20 10 edition) records the pharmacopoeia standard of this Chinese medicine.
4. Latin scientific name Lonicera japonica Thunb (Terminology of Traditional Chinese Medicine (2004))
5 English name "Golden Flower Stem" (Chinese Medicine Terminology (2004))
6. The alias of honeysuckle. It's honeysuckle Honeysuckle. Buxus macrophylla Buxus macrophylla And Erhuamiao [2].
Seven sources of honeysuckle. It's the stems and leaves of Lonicera japonica Thunb. [2].
Honeysuckle. It's a dried stem branch of Lonicera japonica Thunb. [1] of Caprifoliaceae.
It is produced in most areas of China [2].
9 sexual taste is sweet and cold; Into the heart and lung meridian [2].
10 efficacy and indications honeysuckle has the efficacy of clearing away heat and toxic materials and dredging collaterals: treating fever, fever, sores and carbuncle, toxic heat, bloody dysentery and rheumatic arthralgia: decoction, 10 ~ 30g [2].
1 1 chemical constituents Lonicera japonica Thunb leaves contain Lonicera japonica Thunb flavone, Lonicera japonica Thunb glycoside, luteolin, strychnine, etc. The stem contains tannins and alkaloids [2].
Pharmacological effects of luteolin in 12 honeysuckle. It has spasmolytic and anti-inflammatory effects on isolated rabbit intestinal smooth muscle, can inhibit staphylococcus and Bacillus subtilis in vitro, and also has anticancer effect [2].
13 honeysuckle pharmacopoeia standard. 13. 1 is called honeysuckle.
Ren dongteng
Lonicera japonica
13.2 Source This product is the dried stems and branches of Lonicera japonica Thunb. Caprifoliaceae. Harvest in autumn and winter and dry in the sun.
13.3 characteristics this product is long cylindrical, multi-branched, often wound into bundles, with a diameter of1.5 ~ 6mm. The surface is reddish brown to dark brown, and some are grayish green, smooth or fluffy; The skin falls off easily. There are many nodes on the branch, and the internodes are 6 ~ 9 cm long, with residual leaves and leaf scars. Fragile, easy to break, yellow-white cross section, hollow. The gas is weak, the old branches are slightly bitter, and the young branches are light.
13.4 Identification (1) The powder of this product is light brown to yellowish brown. There are many non-glandular hairs, single cells, broken, thick-walled and verrucous processes on the surface. Epidermal cells are brownish yellow to brownish red, with polygonal surface, and often have traces of non-glandular hair shedding, resembling stone cells. There are calcium oxalate clusters in parenchyma cells, which are often arranged in rows, and some are scattered individually, with obtuse edges and rounded corners, with a diameter of 5 ~15 μ m.
(2) Take 65438 0 g of this product powder, add 65438±00ml of 50% methanol, perform ultrasonic treatment for 30 minutes, filter, and take the filtrate as the test solution. In addition, honeysuckle is 65438+/-0g. As a reference medicinal material, the reference medicinal material solution was prepared by the same method. Then take the loganin reference substance and add 50% methanol to make a solution containing 1mg per 1ml as the reference substance solution. According to the thin-layer chromatography test (Appendix ⅵ b), 65438 00μ l of test solution, 65438 μ l of control medicinal material solution and 5μl of control medicinal material solution were respectively spotted on the same silica gel G thin-layer plate, and the lower solution of chloroform-methanol water (65: 35: 10) below 65438 00℃ was used as the developing agent. In the chromatogram of the test sample, spots with the same color appear at the positions corresponding to the chromatograms of the reference medicinal materials and the reference substance.
The moisture content of 13.5.1shall not exceed 12.0% (appendix ⅸ H first method).
13.5.2 the total ash content shall not exceed 4.0% (appendix ⅸ k).
13.6 the extract shall be determined by hot dip method under the item of alcohol-soluble extract determination method (appendix X A), with 50% ethanol as the solvent, which shall not be less than 14.0%.
13.7 content determination 13.7. 1 chlorogenic acid was determined by high performance liquid chromatography (appendix 6 d).
Chromatographic conditions and system applicability test with octadecylsilane bonded silica gel as filler; Using acetonitrile 0.4% phosphoric acid solution (10: 90) as mobile phase; The detection wavelength is 327nm. The theoretical plate number should be no less than 1000 calculated by chlorogenic acid peak.
Preparation of Reference Solution Take an appropriate amount of chlorogenic acid reference substance, weigh it accurately, and add 50% methanol to make a solution containing 40μg per kloc-0/ml.
Preparation of test solution Take about 65438±0g of this product powder (pass through No.3 sieve), weigh it accurately, put it in a conical flask with a stopper, add 25ml of 50% methanol accurately, weigh it, perform ultrasonic treatment (power 250W, frequency 30 kHz) for 30 minutes, let it cool, weigh it again, make up the weightlessness with 50% methanol, and shake it evenly.
The determination method accurately absorbs 65438 00 μ l control solution and 5 ~ 65438 00 μ l test solution respectively, and injects them into liquid chromatograph for determination.
The content of chlorogenic acid (C 16H 18O9) in this product shall not be less than 0. 10% in terms of dry product.
13.7.2 Determination of loganin by HPLC (Appendix VI D).
Chromatographic conditions and system applicability test with phenylsilane bonded silica gel as filler; Acetonitrile 0.4% phosphoric acid solution (12: 88) was used as the mobile phase. The detection wavelength is 236 nm. The theoretical plate number should be not less than 3000 calculated by loganin peak.
Preparation of reference substance solution Take a proper amount of loganin reference substance, weigh it accurately, and add 50% methanol to make a solution containing 40μg per 1ml.
Preparation of test solution Take about 65438±0g of this product powder (pass through No.3 sieve), weigh it accurately, put it in a conical flask with a stopper, add 25ml of 50% methanol accurately, weigh it, perform ultrasonic treatment (power 500w, frequency 40 kHz) for 30 min, let it cool, weigh it again, make up the weight loss with 50% methanol, shake it evenly, and filter it.
The determination method accurately absorbs 65438 00 μ l of the control solution and 2 ~ 65438 00 μ l of the test solution, respectively, and injects them into the liquid chromatograph for determination.
The content of loganin (C 17H26O 10) shall not be less than 0. 10% in terms of dry products.
13.8 honeysuckle decoction pieces 13.8. 1 processing to remove impurities, cleaning, moistening intestines, cutting into sections and drying.
This product presents irregular fragments. The surface is brownish red (twigs), some are grayish green, smooth or fluffy; The skin falls off easily. The section is yellow and white, hollow. Occasionally there are residual leaves, dark green and slightly fluffy. The gas is weak, the old branches are slightly bitter, and the young branches are light.
The content determination of 13.8.2 is the same as that of medicinal materials, and the content of chlorogenic acid (C 16H 18O9) is less than 0.070%.
13.8.3 Identification, inspection, extract and content determination (loganin) are the same as those of the medicinal materials.
13.8.4 Sexual taste and sweet and cold meridian tropism. Enter the lung and stomach meridians.
13.8.5 Functions: clearing away heat and toxic materials, expelling wind and dredging collaterals. Can be used for treating fever, bloody dysentery due to heat toxin, carbuncle, swelling, sore, rheumatism, arthralgia, swelling and heat pain.
13.8.6 Usage and dosage 9 ~ 30g.
13.8.7 Store in a dry place.
13.9 source: People's Republic of China (PRC) Pharmacopoeia (20 10).
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